Shodex ion-exchange and ion-exclusion HPLC columns

Shodex Ion-Exchange and Ion-Exclusion HPLC Columns

Shodex ion-exchange and ion-exclusion chromatography columns provide a comprehensive solution for the high-precision separation of carbohydrates, organic acids, ionic and neutral compounds, and biomolecules. Shodex polymer-based columns provide long service life, excellent reproducibility and stable operation for routine testing as well as analyses in research and regulated environments.

Ligand-exchange chromatography columns– Ligand Exchange Chromatography Columns

Shodex ligand-exchange HPLC columns achieve separation through a combination of the ligand-exchange mechanism and, in some cases, size-exclusion or HILIC effects. As a result, they provide excellent selectivity and resolution, even for analytes with very similar structures.

Ligand exchange + size-exclusion columns

These columns are primarily suitable for separating mono-, di- and oligosaccharides, as well as polysaccharides. For many types, pure water is sufficient as the eluent, enabling straightforward method development and stable detection.

Key benefits:

separation of sugars using ligand exchange + size-exclusion mechanisms
aqueous eluent only for certain types
excellent reproducibility
suitable for routine testing and standard methods

Typical applications:

glucose, fructose, sucrose, maltose
lactose, lactulose
oligosaccharides, starch hydrolysates
biomass and fermentation samples

Ligand exchange + HILIC columns

The combination of ligand-exchange and HILIC mechanisms enables the effective separation of neutral and slightly ionic sugars and sugar alcohols using eluents that also contain organic solvents.

Benefits:

improved selectivity for complex sugar mixtures
broader method development options

Applications:

saccharides and sugar alcohols
taurine and related compounds
food additives (e.g. sweeteners)
Columns suitable for pharmacopoeial methods

Several Shodex ligand-exchange column types are for USP, JP and EP pharmacopoeial methods validated, making them an ideal choice for analyses in regulated environments and quality-control testing.

Typical applications:

sorbitol, mannitol, maltitol
lactose and related sugars
pharmaceutical excipients
QC and validated analytical methods

Shodex Ion Exclusion chromatography columns

Shodex Ion Exclusion chromatography columns have been specifically developed for the reliable separation of organic acids, sugars, sugar alcohols and alcohols. The ion-exclusion mechanism is particularly effective for analysing weak acids and neutral components where ion-exchange or reversed-phase techniques do not provide satisfactory results.

Key applications:

organic acids
sugars and sugar alcohols
fermentation samples
wine, beer and other food products
alcohols and residual solvents
glycerol, glycols, furan derivatives

Benefits:

stable operation with acidic eluents
effective with isocratic methods
ideal for routine analyses and validated methods

Shodex Ion Chromatography columns – anion analysis

Shodex ion chromatography columns designed for anion analysis are widely applicable to the determination of inorganic and organic anions in drinking water, environmental samples and industrial water. The columns are available for both suppressed and non-suppressed systems.

Components that can be analysed:

fluoride, chloride, bromide, iodide
nitrate, nitrite, sulphate, phosphate
oxyanions
organic acids and their derivatives

Technical features:

rapid analysis
consistent peak shape even at low concentrations
compatible with drinking-water and environmental analysis standards

Shodex Ion Chromatography columns – cation analysis

Shodex ion chromatography columns for cation analysis provide a reliable solution for separating alkali and alkaline-earth metals, ammonium, amines and transition metals.

Typical applications: