Generon PATHfinder – Real-Time PCR kits for rapid detection of pathogenic microorganisms

The Generon PATHfinder product range offers advanced Real-Time PCR solutions for the rapid, sensitive and reliable detection of pathogenic microorganisms in food, water, beverage and environmental samples. The kits enable molecular identification of key bacteria, parasites, spoilage microorganisms and virulence genes, significantly reducing the time required compared with conventional microbiological testing.

The PATHfinder portfolio includes singleplex and multiplex (2-plex, 3-plex and 4-plex) Real-Time PCR kits, serotyping assays, virulence gene analysis and positive controls. Many assays have been developed in accordance with international standards, including ISO 10272, ISO 12869, ISO 13136, ISO 17919 and ISO 18867, making them an excellent choice for food industry, water testing, environmental analysis and research laboratories.

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Available PATHfinder assays

Testing area	Description
Detection of foodborne bacteria	Rapid Real-Time PCR assays for detecting key foodborne pathogens, such as Salmonella, Listeria, Campylobacter, Cronobacter, Staphylococcus aureus, Clostridium and Yersinia species.
STEC and E. coli assays	Detection of Shiga toxin-producing Escherichia coli (STEC/VTEC), virulence gene testing and serotyping in accordance with ISO 13136 recommendations.
Water microbiology testing	Rapid molecular detection of Legionella spp., Legionella pneumophila and other waterborne pathogens in drinking water and environmental samples.
Parasite detection	PCR kits for detecting parasites such as Giardia, Cryptosporidium and Anisakis in food and environmental samples.
Beverage and wine microbiology	Specialised PCR kits for detecting beverage spoilage microorganisms such as Alicyclobacillus and Brettanomyces.
Multiplex PCR assays	2-, 3- and 4-plex Real-Time PCR kits enabling the simultaneous detection of multiple microorganisms or target genes.
Positive controls and validation reagents	Positive controls and supplementary reagents for assay quality assurance and method validation.

The Generon PATHfinder portfolio enables microbiology laboratories to rapidly and reliably detect key foodborne pathogens, waterborne microorganisms and specialised testing targets using Real-Time PCR.

Foodborne pathogens	Water microbiology	Specialised assays
Salmonella spp.	Legionella spp.	Alicyclobacillus spp.
Listeria spp.	Legionella pneumophila	Brettanomyces
Listeria monocytogenes	Pseudomonas aeruginosa	Cryptosporidium
STEC / VTEC		Giardia
Shigella spp.		Anisakis
Campylobacter spp.		Pseudoterranova
Staphylococcus aureus
Clostridium perfringens
Clostridium botulinum
Cronobacter spp.
Yersinia spp.
Vibrio spp.
Bacillus cereus group

Advantages of PATHfinder Real-Time PCR kits

Rapid and reliable Real-Time PCR-based detection
Singleplex and multiplex (2-, 3- and 4-plex) assays
For testing food, water, beverage and environmental samples
Kits also suitable for identifying virulence genes and serotypes
Support for testing in accordance with numerous ISO standards
Positive controls and validation reagents available
Ideal for food industry, water testing, environmental analysis and research laboratories

Reference materials, positive controls and process controls

In addition to Real-Time PCR detection kits, the Generon PATHfinder portfolio also offers reference DNA, positive controls and SureXtra process controls to support the validation of PCR assays, laboratory quality assurance and verification of test result reliability.

Reference materials and controls enable continuous monitoring and verification of the entire analytical process, from DNA extraction to Real-Time PCR measurement, making them particularly recommended for accredited laboratories, quality control laboratories and research institutes.

Available control and reference materials