HILICON – specialised HILIC columns

HILICON – specialised HILIC columns

iHILIC HILIC HPLC and UHPLC columns – HILICON

iHILIC columns are developed by HILICON AB and specifically optimised for the HILIC (Hydrophilic Interaction Liquid Chromatography) separation of polar compounds. The product range can be used with both UHPLC and HPLC systems and offers solutions particularly suited to LC-MS.

The columns use zwitterionic, charge-modulated amide and diol functional groups, providing excellent selectivity, stability and exceptionally low bleed.

Key benefits:

• efficient HILIC separation of polar compounds
• complementary selectivities for broader method-development options
• excellent durability and ultra-low bleed
• LC-MS compatible, ideal for “omics” applications
• stable performance on both UHPLC and HPLC systems

iHILIC products

iHILIC –Fusion (silica -based)

• Balanced HILIC selectivity
• Available particle sizes: 1.8 / 3.5 / 5 µm
• pH range: 2–8
• Highly recommended for LC-MS-based analyses

iHILIC –Fusion (+) (silica -based)

• Modified charge distribution for finer selectivity
• An excellent choice for complex polar mixtures
• UHPLC and HPLC compatible

iHILIC –Fusion(P) (polymer-based)

• Enhanced chemical and pH stability
• pH range: 1–10
• Long service life, even with more aggressive eluents

iHILIC -(P) Classic (polymer-based)

• For classic HILIC separations
• Stable performance across a broad range of applications
• 5 µm particle size, pH 1–10

Typical application areas:

• metabolomics, proteomics
• polar small molecules
• nucleotides, nucleosides
• sugars, amino acids
• active pharmaceutical ingredients and impurities
• LC-MS-based “omics” studies

Summary

iHILIC columns offer a reliable, modern solution for the high-resolution separation of polar compounds, whether for routine testing, research and development or LC-MS applications. Silica- and polymer-based versions enable optimal method selection for every analytical requirement.