Shodex Reversed-Phase and HILIC HPLC Columns

Shodex Reversed-Phase and HILIC HPLC Columns

Shodex polymer-based reversed-phase HPLC columns (Polymer-based Reversed Phase)

Polymer-based reversed-phase HPLC columns are an excellent alternative to conventional silica-based ODS columns, particularly when a wide pH range, high alkali resistance or analysis in a biological matrix is required.

These columns use a polymeric packing material that is not susceptible to hydrolysis, providing a longer service life, more stable retention and better sample recovery.

ODP2 HP – highly alkali-resistant polymeric RP columns

ODP2 HP columns offer exceptional alkali tolerance and nearly twice the theoretical plate count of other polymeric RP columns.

Key benefits:

• excellent performance at high pH (e.g. protein or serum samples)
• improved retention of small and highly polar molecules
• suitable for LC/MS analysis
• complies with USP-NF L39 requirements

Typical applications:

• active pharmaceutical ingredients in biological matrices
• imidazoles, local anaesthetics
• quantitative measurements in the presence of proteins

Asahipak ODP – classic polymeric RP solutions

Asahipak ODP columns have a larger pore size, making them particularly suitable for analysing amino acids, peptides and proteins.

Features:

• wide pH range (pH 2–13)
• can be used with 100% water and buffer solutions
• excellent stability over long operating periods
• complies with USP-NF L67 requirements

RSpak – specialised polymeric RP columns

The RSpak column family is specifically designed for applications where:

• conventional ODS columns do not retain the sample,
• or neutral and ionic components need to be analysed together.

Applications:

• organic acids, vitamins,
• food additives,
• amino acids, complex biological samples

Polymer-based HILIC columns (Hydrophilic Interaction Chromatography)

HILIC chromatography is an ideal solution for separating highly polar compounds that are poorly retained or not retained at all in reversed-phase chromatography.

The benefits of polymer-based HILIC columns include:

• excellent chemical stability
• long service life
• low degradation

HILICpak – high-sensitivity polymeric HILIC columns

HILICpak columns are available with various functional groups:

• amino, quaternary ammonium and carboxyl

Applications:

• sugars and oligosaccharides
• nucleotides
• phosphates
• high-sensitivity LC/MS measurements

Particularly suitable for:

• ELSD, CAD and LC/MS detectors
• measurements at low flow rates

Asahipak HILIC columns

The Asahipak NH2 series is a classic, reliable HILIC solution offering:

• a high theoretical plate count
• excellent reproducibility
• compliance with USP-NF L82 requirements

An ideal choice for analysing:

• saccharides, sugar alcohols,
• polar metabolites.

Silica-based reversed-phase HPLC columns (ODS)

Silica-based ODS columns for routine HPLC analyses.

C18M – classic ODS columns

• Monomeric C18 phase
• High-purity silica (≥99.9%)
• Fully end-capped design
• Complies with the USP-NF L1 standard

UHPLC ODS columns

ODS columns designed for UHPLC:

• are manufactured with particle sizes below 1.9 µm
• can withstand pressures up to 100 MPa
• offer excellent mechanical and alkaline stability

An ideal choice for:

• rapid separations
• high-resolution analyses
• modern UHPLC systems

Wide selection – catalogue-based search

Polymer- and silica-based RP and HILIC columns are available in hundreds of configurations (dimensions, pore size, particle size and functionalisation), so the full range is not listed individually.